For TaqMan assays, keep amplicons between 60–150 bp. For SYBR Green, 75–200 bp. The software defaults can be adjusted in the “Design Parameters” tab.
The registration process is a critical point for users, as the software requires a unique code for activation: Finding the Code : The registration code is typically found on the Primer Express CD envelope or accompanying paperwork. Activation primer express 30 1 registration code top
: A cost-effective option for larger research teams or core facilities. Key Features of Version 3.0.1 For TaqMan assays, keep amplicons between 60–150 bp
Once you have a legitimate registration, here are expert tips to get top results: The registration process is a critical point for
Typically, no. The single-user license is tied to one named user. A lab-wide license is required for multiple users. Check your EULA.
Primer Express™ Software v3.0.1 is a specialized primer and probe design tool developed by Applied Biosystems (now part of Thermo Fisher Scientific ) for real-time PCR applications. This software is essential for researchers designing assays for gene quantification and allelic discrimination using TaqMan and SYBR™ Green I dye chemistries. How to Register Primer Express 3.0.1